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The introduction of the cell culture technique in the 1980s, through which disparate cell sorts may cohabit in a petri dish bathed in a supportive medium, proved important. The co-culturing of neurones and glia led to the first important discovery, namely that neurones survive higher in tradition when astrocytes are additionally current (Whatley et al., buy healthy flow blood 1981). This preliminary research did not reveal what side of the presence of astrocytes the neurones discovered important for survival. This was not a trivial matter because the support afforded by astrocytes could possibly be physical, the place connections between cells are essential, or the release of some trophic factor from astrocytes that supported the neurones. A later tradition study revealed that the essential element of astrocyte presence that supported neurones was the glycogen contained throughout the astrocytes (Swanson, 1992; Swanson and Choi, 1993). Astrocytes depleted of glycogen weren't as profitable at supporting neurones as astrocytes with a full complement of glycogen.

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